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KMID : 0368419910340040261
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Journal of Plant Biology
1991 Volume.34 No. 4 p.261 ~ p.266
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Gene Expression in Transformed Higher Plants
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鄭賢淑/Cheong, Hyeon Sook
金玲希/表炳植/金成俊/康榮熹/Kim, Young Hee/Pyo, Byoung Sik/Kim, Sung Jun/Kang, Young Hee
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Abstract
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The hairy root was induced from potato tuber disc by infection of A. rhizogenes. The detection of the agropine and mannopine by paper electrophoresis confirmed that induced hairy root was transformed by A. rhizogenes. The activity of peroxidase was the highest at 5 weeks and isozyme pattern of peroxidase revealed 3 cathodic bands and 2 anodic bands and new C_4 band (pI 4.6) was observed at 7 weeks after cultivation in hairy root with isoelectric focusing. To study the effect of Ca^2+ on cell wall formation in hairy root, channel blocker of Ca^2+ was treated. The activity of β-glucan synthetase Ⅱ (GS Ⅱ) related to cell wall synthesis was inhibited by about 50% in diltiazem and flunarizine treatment than that of control, but stimulated in CaCl_2 treatment. Therefore these results showed that Ca^2+ might be and effective factor in the cell wall formation. The activity of GS Ⅱ by NaF treatment was increased by about 30%. This result suggested that the activity of GS Ⅱ is changed through phosphorylation process.
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